SynonymsKLF10,EGRA,TIEG,Krueppel-Like Factor 10,TGFB Inducible Early Growth Response,EGR-alpha,Transforming growth factor-beta-inducible early growth response protein 1
ReactivityHuman
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
HUDL02895
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13118
Sensitivity:
0.122ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
KLF10,EGRA,TIEG,Krueppel-Like Factor 10,TGFB Inducible Early Growth Response,EGR-alpha,Transforming growth factor-beta-inducible early growth response protein 1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human TGF Beta Inducible Early Response Gene 1 (TIEG1) ELISA Kit
The Human TGF-Beta Inducible Early Response Gene 1 (TIEG1) ELISA Kit is a reliable tool for the precise measurement of TIEG1 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its excellent sensitivity and specificity, this kit delivers accurate and consistent results, making it suitable for a diverse range of research investigations.TIEG1 is a key protein involved in various cellular processes, including cell differentiation, cell cycle regulation, and apoptosis. Its dysregulation has been implicated in multiple diseases, including cancer, inflammatory disorders, and metabolic diseases.
Therefore, TIEG1 serves as a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Designed to meet the needs of researchers in the fields of molecular biology, immunology, and cell biology, the Human TIEG1 ELISA Kit from Assay Genie offers a convenient and efficient solution for analyzing TIEG1 expression levels in human samples. Get accurate and reliable results with this ELISA kit to advance your research and contribute to a deeper understanding of TIEG1 biology and its implications in human health and disease.
Product Name:
Human TGF Beta Inducible Early Response Gene 1 (TIEG1) ELISA Kit
Product Code:
HUDL02895
Size:
96 Assays
Synonyms:
KLF10, EGRA, TIEG, Krueppel-Like Factor 10, TGFB Inducible Early Growth Response, EGR-alpha, Transforming growth factor-beta-inducible early growth response protein 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
