The IER5 Antibody (CAB18457) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific to human samples and is validated for use in Western blot applications, allowing for the detection and analysis of IER5 in various cell types.IER5, also known as immediate early response 5, plays a crucial role in immune regulation and cellular stress response. It is implicated in processes such as cell cycle control, apoptosis, and DNA damage repair, making it a key target for research in fields such as immunology, oncology, and neurology.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IER5 Antibody
SKU:
CAB18457
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SBBI48, IER5
Positive Sample:
HeLa, A-549, Mouse testis, Rat lung
Cellular Localization:
Cytoplasm, Nucleolus, Nucleoplasm, Nucleus.
Calculated MW:
34kDa
Observed MW:
34kDa
This gene encodes a protein that is similar to other immediate early response proteins. In the mouse, a similar gene may play an important role in mediating the cellular response to mitogenic signals. Studies in rats found the expression of a similar gene to be increased after waking and sleep deprivation.
Purification Method
Affinity purification
Gene ID
51278
RRID
AB_2862224
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using IER5 Rabbit pAb (CAB18457) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human esophageal using IER5 Rabbit pAb (CAB18457) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
