Anti-IGF2BP2 Antibody (CAB1774)

Antibody Name:	Anti-IGF2BP2 Antibody
Antibody SKU:	CAB1774
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 330-599 of human IGF2BP2 (NP_006539.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, BxPC-3, HeLa, Jurkat, NIH/3T3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 330-599 of human IGF2BP2 (NP_006539.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TVEA CASA EIEI MKKL REAF ENDM LAVN QQAN LIPG LNLS ALGI FSTG LSVL SPPA GPRG APPA APYH PFTT HSGY FSSL YPHH QFGP FPHH HSYP EQEI VNLF IPTQ AVGA IIGK KGAH IKQL ARFA GASI KIAP AEGP DVSE RMVI ITGP PEAQ FKAQ GRIF GKLK EENF FNPK EEVK LEAH IRVP SSTA GRVI GKGG KTVN ELQN LTSA EVIV PRDQ TPDE NEEV IVRI IGHF FASQ TAQR KIRE IVQQ VKQQ EQKY PQGV ASQR SK
Gene ID:	10644
Uniprot:	Q9Y6M1
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	54kDa/58kDa/59kDa/61kDa/66kDa
Observed MW:	70kDa

Synonyms:	IGF2BP2, IMP-2, IMP2, VICKZ2
Background:	This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternative promoter usage and alternate splicing result in multiple variants encoding different isoforms.

UniProt Protein Function:	IGF2BP2: Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. Belongs to the RRM IMP/VICKZ family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; Translation Chromosomal Location of Human Ortholog: 3q27.2 Cellular Component: cytoskeleton; cytoplasm; nucleus; cytosol Molecular Function:protein binding; mRNA 3'-UTR binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding Biological Process: anatomical structure morphogenesis; mRNA transport; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process Disease: Diabetes Mellitus, Noninsulin-dependent
NCBI Summary:	This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternative promoter usage and alternate splicing result in multiple variants encoding different isoforms. [provided by RefSeq, May 2014]
UniProt Code:	Q9Y6M1
NCBI GenInfo Identifier:	224471831
NCBI Gene ID:	10644
NCBI Accession:	Q9Y6M1.2
UniProt Secondary Accession:	Q9Y6M1,A0A4Z0, B3FTN2, B3FTN3, B3FTN4,
UniProt Related Accession:	Q9Y6M1
Molecular Weight:	599
NCBI Full Name:	Insulin-like growth factor 2 mRNA-binding protein 2
NCBI Synonym Full Names:	insulin-like growth factor 2 mRNA binding protein 2
NCBI Official Symbol:	IGF2BP2
NCBI Official Synonym Symbols:	IMP2; IMP-2; VICKZ2
NCBI Protein Information:	insulin-like growth factor 2 mRNA-binding protein 2; VICKZ family member 2; IGF2 mRNA-binding protein 2; IGF-II mRNA-binding protein 2
UniProt Protein Name:	Insulin-like growth factor 2 mRNA-binding protein 2
UniProt Synonym Protein Names:	Hepatocellular carcinoma autoantigen p62; IGF-II mRNA-binding protein 2; VICKZ family member 2
Protein Family:	Insulin-like growth factor 2 mRNA-binding protein
UniProt Gene Name:	IGF2BP2
UniProt Entry Name:	IF2B2_HUMAN

	Western blot analysis of extracts of various cell lines, using IGF2BP2 antibody (CAB1774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U-2 OS cells using IGF2BP2 antibody (CAB1774) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of rat testis cells using IGF2BP2 antibody (CAB1774) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-IGF2BP2 Antibody (CAB14103)

Anti-IGF2BP2 Antibody (CAB2749)

Anti-IMP2 Antibody (CAB5189)

IGF2BP2 Antibody (PACO02350)

IGF2BP2 Antibody (PACO09892)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)