Anti-IL-3RA Antibody (CAB3926)

Antibody Name:	IL3RA Rabbit Polyclonal Antibody
Antibody SKU:	CAB3926
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IL3RA (NP_002174.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IL3RA (NP_002174.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVLL WLTL LLIA LPCL LQTK EDPN PPIT NLRM KAKA QQLT WDLN RNVT DIEC VKDA DYSM PAVN NSYC QFGA ISLC EVTN YTVR VANP PFST WILF PENS
Gene ID:	3563
Uniprot:	P26951
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	34kDa/43kDa
Observed MW:	70kDa

Synonyms:

IL3RA, CD123, IL3R, IL3RAY, IL3RX, IL3RY, hIL-3Ra

Background:

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.

UniProt Protein Function:	IL3RA: This is a receptor for interleukin-3. Belongs to the type I cytokine receptor family. Type 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: Xp22.3 or Yp11.3 Cellular Component: plasma membrane; integral to membrane Molecular Function:interleukin-3 receptor activity
NCBI Summary:	The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]
UniProt Code:	P26951
NCBI GenInfo Identifier:	417184
NCBI Gene ID:	3563
NCBI Accession:	P26951.1
UniProt Related Accession:	P26951
Molecular Weight:
NCBI Full Name:	Interleukin-3 receptor subunit alpha
NCBI Synonym Full Names:	interleukin 3 receptor subunit alpha
NCBI Official Symbol:	IL3RA
NCBI Official Synonym Symbols:	IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
NCBI Protein Information:	interleukin-3 receptor subunit alpha
UniProt Protein Name:	Interleukin-3 receptor subunit alpha
UniProt Synonym Protein Names:	CD_antigen: CD123
Protein Family:	Interleukin-3 receptor
UniProt Gene Name:	IL3RA
UniProt Entry Name:	IL3RA_HUMAN

	Western blot analysis of extracts of A-549 cells, using IL3RA antibody (CAB3926) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using IL3RA antibody (CAB3926) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using IL3RA antibody (CAB3926) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Human CD123 Monoclonal Antibody [6H6](AGEL0443)	Human IL-3RA ELISA Kit (HUFI02103)
Human CD123 Monoclonal Antibody (Biotin Conjugated) [6H6](AGEL0444)	Human IL-3RA ELISA Kit
Human CD123 Monoclonal Antibody (Alexa Fluor 488 Conjugated) [6H6](AGEL0445)
Human CD123 Monoclonal Antibody (FITC Conjugated) [6H6](AGEL1455)
Human CD123 Monoclonal Antibody (PE Conjugated) [6H6](AGEL1456)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)