K63-linkage Specific Polyubiquitin Antibody (CAB18164)
The K63-linkage Specific Polyubiquitin Antibody (CAB18164) is a high-quality antibody developed for reliable detection and analysis of target proteins. Ubiquitination,onetypeofthemostcommonpost-translationalmodification,mediatestheregulationofproteinhomeostasisinvivo.SinceubiquitinitselfcontainsmultiplelysineresiduesandoneN-terminalfreeaminogroup,eighttypesofubiquitinchainscanbeformed.? K63-linked polyubiquitin chains exert nonproteolytic functions in vivo, such as protein trafficking, kinase/phosphatase activation, and DNA damage control, all of which might be important in regulation of cancer survival and development （PMID: 19217402，PMCID: PMC3008764）.
This antibody is validated for use in ELISA, DB applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
K63-linkage Specific Polyubiquitin Antibody
SKU:
CAB18164
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
ELISADB
Recommended Dilution:
DB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
HeLa, HeLa+MG132, NIH/3T3, C6, Mouse brain, Rat brain
Observed MW:
15-200kDa
Ubiquitination,onetypeofthemostcommonpost-translationalmodification,mediatestheregulationofproteinhomeostasisinvivo.SinceubiquitinitselfcontainsmultiplelysineresiduesandoneN-terminalfreeaminogroup,eighttypesofubiquitinchainscanbeformed.? K63-linked polyubiquitin chains exert nonproteolytic functions in vivo, such as protein trafficking, kinase/phosphatase activation, and DNA damage control, all of which might be important in regulation of cancer survival and development （PMID: 19217402，PMCID: PMC3008764）.
Purification Method
Affinity purification
RRID
AB_2861949
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Dot-blot analysis of all sorts of peptides using K63-linkage Specific Polyubiquitin antibody (CAB18164) at 1:1000 dilution.
