Anti-KCNMB2 Antibody (CAB10277)

Antibody Name:	Anti-KCNMB2 Antibody
Antibody SKU:	CAB10277
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 68-194 of human KCNMB2 (NP_005823.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse brain, Mouse lung, Rat brain, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 68-194 of human KCNMB2 (NP_005823.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TLLR SYMQ SVWT EESQ CTLL NASI TETF NCSF SCGP DCWK LSQY PCLQ VYVN LTSS GEKL LLYH TEET IKIN QKCS YIPK CGKN FEES MSLV NVVM ENFR KYQH FSCY SDPE GNQK SVIL TKLY SSN
Gene ID:	10242
Uniprot:	Q9Y691
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	27kDa
Observed MW:	30kDa

Synonyms:

KCNMB2

Background:

MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which decreases the activation time of MaxiK alpha subunit currents. Alternative splicing results in multiple transcript variants of this gene. Additional variants are discussed in the literature, but their full length nature has not been described.

UniProt Protein Function:	KCNMB2: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Acts as a negative regulator that confers rapid and complete inactivation of KCNMA1 channel complex. May participate in KCNMA1 inactivation in chromaffin cells of the adrenal gland or in hippocampal CA1 neurons. Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB2 subfamily.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 3q26.32 Cellular Component: integral to plasma membrane; plasma membrane; voltage-gated potassium channel complex Molecular Function:calcium-activated potassium channel activity; ion channel inhibitor activity; potassium channel regulator activity Biological Process: detection of calcium ion; generation of action potential; potassium ion transport; regulation of action potential; regulation of vasoconstriction; synaptic transmission
NCBI Summary:	MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which decreases the activation time of MaxiK alpha subunit currents. Alternative splicing results in multiple transcript variants of this gene. Additional variants are discussed in the literature, but their full length nature has not been described. [provided by RefSeq, Jul 2013]
UniProt Code:	Q9Y691
NCBI GenInfo Identifier:	46396054
NCBI Gene ID:	10242
NCBI Accession:	Q9Y691.1
UniProt Related Accession:	Q9Y691
Molecular Weight:	27kDa
NCBI Full Name:	Calcium-activated potassium channel subunit beta-2
NCBI Synonym Full Names:	potassium calcium-activated channel subfamily M regulatory beta subunit 2
NCBI Official Symbol:	KCNMB2
NCBI Protein Information:	calcium-activated potassium channel subunit beta-2
UniProt Protein Name:	Calcium-activated potassium channel subunit beta-2
UniProt Synonym Protein Names:	BK channel subunit beta-2; BKbeta2; Hbeta2; Calcium-activated potassium channel, subfamily M subunit beta-2; Charybdotoxin receptor subunit beta-2; Hbeta3; K(VCA)beta-2; Maxi K channel subunit beta-2; Slo-beta-2
Protein Family:	Calcium-activated potassium channel
UniProt Gene Name:	KCNMB2

	Western blot analysis of extracts of various cell lines, using KCNMB2 antibody (CAB10277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human appendix using KCNMB2 antibody (CAB10277) at dilution of 1:100 (40x lens).

Antibodies

KCNMB2 Antibody (PACO01049)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)