Western blot analysis of extracts from wild type(WT) and FOSL1 knockdown (KD) U-87MG cells, using FOSL1 antibody (CAB21988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The [KD Validated] FOSL1 Polyclonal Antibody (CAB21988) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been rigorously validated for use in various applications, including Western blotting, immunohistochemistry, and flow cytometry.Fosl1 plays a crucial role in regulating gene expression and cell proliferation, making it a key player in pathways related to cancer, wound healing, and tissue regeneration.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KD Validated] FOSL1 Polyclonal Antibody
SKU:
CAB21988
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FRA, FRA1, fra-1, [KD Validated] FOSL1
Positive Sample:
U-87MG
Cellular Localization:
Nucleus.
Calculated MW:
29kDa
Observed MW:
40kDa
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
8061
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type(WT) and FOSL1 knockdown (KD) U-87MG cells, using [KD Validated] FOSL1 Rabbit pAb (CAB21988) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
