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Anti-KDM4B Antibody (CAB5824)

SKU:
CAB5824
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-KDM4B Antibody CAB5824
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-KDM4B Antibody CAB5824
€139 - €419
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Description

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Antibody Name:Anti-KDM4B Antibody
Antibody SKU:CAB5824
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human KDM4B (NP_055830.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:BT-474, Jurkat, 293T, NIH/3T3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human KDM4B (NP_055830.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGSE DHGA QNPS CKIM TFRP TMEE FKDF NKYV AYIE SQGA HRAG LAKI IPPK EWKP RQTY DDID DVVI PAPI QQVV TGQS GLFT QYNI QKKA MTVG EYRR LANS EKYC TPRH QDFD DLER KYWK NLTF VSPI YGAD ISGS LYDD DVAQ WNIG SLRT ILDM VERE CGTI IEGV NTPY LYFG MWKT TFAW HTED MDLY SINY LHFG EPKS WYAI PPEH GKRL ERLA IGFF PGSS QGCD AFLR HKMT LISP IILK KYGI PFSR
Gene ID:23030
Uniprot:O94953
Cellular Location:Nucleus
Calculated MW:50kDa/121kDa
Observed MW:110kDa
Synonyms:KDM4B, JMJD2B, TDRD14B
Background:
UniProt Protein Function:JMJD2B: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate. Belongs to the JHDM3 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.14.11.-; Oxidoreductase; Demethylase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function:histone demethylase activity

UniProt Code:O94953
NCBI GenInfo Identifier:134047803
NCBI Gene ID:23030
NCBI Accession:O94953.4
UniProt Secondary Accession:O94953,O75274, Q6P3R5, Q9P1V1, Q9UF40, B9EGN8, D6W631
UniProt Related Accession:O94953
Molecular Weight:50,582 Da
NCBI Full Name:Lysine-specific demethylase 4B
NCBI Synonym Full Names:lysine demethylase 4B
NCBI Official Symbol:KDM4B  
NCBI Official Synonym Symbols:JMJD2B; TDRD14B  
NCBI Protein Information:lysine-specific demethylase 4B
UniProt Protein Name:Lysine-specific demethylase 4B
UniProt Synonym Protein Names:JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B
Protein Family:Lysine-specific demethylase
UniProt Gene Name:KDM4B  
UniProt Entry Name:KDM4B_HUMAN
Anti-KDM4B Antibody (CAB5824)Western blot analysis of extracts of various cell lines, using KDM4B antibody (CAB5824) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
Anti-KDM4B Antibody (CAB5824)Immunofluorescence analysis of U2OS cells using KDM4B antibody (CAB5824). Blue: DAPI for nuclear staining.

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