The [KO Validated] KMT5C Antibody (CAB16235) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting KMT5C in human samples, making it a valuable asset for researchers conducting Western blot experiments.KMT5C, also known as lysine methyltransferase 5C, is involved in modifying histone proteins, thereby influencing gene transcription and cell function. Aberrant KMT5C activity has been linked to various diseases, including cancer and developmental disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] KMT5C Antibody
SKU:
CAB16235
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
FLPE SGFT ILPC TRYS METN GAKI VSTR AWKK NEKL ELLV GCIA ELRE ADEG LLRA GEND FSIM YSTR KRSA QLWL GPAA FINH DCKP NCKF VPAD GNAA C
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SUV420H2, Suv4-20h2, 5C
Positive Sample:
HeLa, 293T, Jurkat, Mouse thymus
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
52kDa
Observed MW:
52kDa
SUV420H2 and the related enzyme SUV420H1 (MIM 610881) function as histone methyltransferases that specifically trimethylate nucleosomal histone H4 (see MIM 602822) on lysine-20 (K20) (Schotta et al., 2004 [PubMed 15145825]).
Purification Method
Affinity purification
Gene ID
84787
RRID
AB_2770122
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] KMT5C Rabbit pAb (CAB16235) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from Mouse thymus, using [KO Validated] KMT5C Rabbit pAb (CAB16235) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
