Western blot analysis of extracts of various cell lines, using MCM6 antibody (CAB21112) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The [KO/KD Validated] MCM6 Monoclonal Antibody (CAB21112) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, specifically designed for use in knockout studies, is highly specific to the MCM6 protein, a key component of the DNA replication licensing machinery.The antibody, rigorously tested and validated in knockout cell lines, is ideal for Western blotting and immunofluorescence applications in a variety of cell types. Its exceptional specificity and sensitivity make it a valuable asset for investigations into cell cycle regulation, DNA replication dynamics, and mechanisms of genomic stability.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO/KD Validated] MCM6 Monoclonal Antibody
SKU:
CAB21112
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC3057
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Mis5, P105MCM, MCG40308, [KO/KD Validated] MCM6
Positive Sample:
293T, HeLa, Jurkat, Mouse testis
Cellular Localization:
Nucleus.
Calculated MW:
93kDa
Observed MW:
93kDa
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood.
Purification Method
Affinity purification
Gene ID
4175
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KD Validated] MCM6 Rabbit mAb (CAB21112) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from wild type (WT) and MCM6 knockdown (KD) 293T cells, using [KD Validated] MCM6 Rabbit mAb (CAB21112) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
