Anti-ATG4D Antibody (CAB18097)

Antibody Name:	Anti-ATG4D Antibody [KO Validated]
Antibody SKU:	CAB18097
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ATG4D (NP_116274.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ATG4D (NP_116274.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNSV SPAA AQYR SSSP EDAR RRPE ARRP RGPR GPDP NGLG PSGA SGPA LGSP GAGP SEPD EVDK FKAK FLTA WNNV KYGW VVKS RTSF SKIS SIHL CGRR YRFE GEGD IQRF QRDF VSRL WLTY RRDF PPLP GGCL TSDC GWGC MLRS GQMM LAQG LLLH FLPR DWTW AEGM GLGP PELS GSAS PSRY HGPA RWMP PRWA QGAP ELEQ ERRH RQIV SWFA DHPR APFG LH
Gene ID:	84971
Uniprot:	Q86TL0
Cellular Location:	Cytoplasm, Mitochondrion matrix
Calculated MW:	16kDa/52kDa
Observed MW:	53kDa

Anti-ATG4D Antibody [KO Validated] (CAB18097)

Western blot analysis of extracts from normal (control) and ATG4D knockout (KO) HeLa cells, using ATG4D antibody (CAB18097) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.

Synonyms:

ATG4D, APG4-D, APG4D, AUTL4

Background:

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ATG4D: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Belongs to the peptidase C54 family.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; Protease; EC 3.4.22.-; Autophagy Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: cytoplasm; cytosol Molecular Function:cysteine-type endopeptidase activity Biological Process: autophagic vacuole formation; C-terminal protein lipidation; mitochondrion degradation; protein delipidation; protein processing; protein targeting to membrane
NCBI Summary:	Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:	Q86TL0
NCBI GenInfo Identifier:	61211809
NCBI Gene ID:	84971
NCBI Accession:	Q86TL0.1
UniProt Secondary Accession:	Q86TL0,Q969K0,
UniProt Related Accession:	Q86TL0
Molecular Weight:	16,008 Da
NCBI Full Name:	Cysteine protease ATG4D
NCBI Synonym Full Names:	autophagy related 4D cysteine peptidase
NCBI Official Symbol:	ATG4D
NCBI Official Synonym Symbols:	APG4D; AUTL4; APG4-D
NCBI Protein Information:	cysteine protease ATG4D
UniProt Protein Name:	Cysteine protease ATG4D
UniProt Synonym Protein Names:	AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D
Protein Family:	Cysteine protease
UniProt Gene Name:	ATG4D
UniProt Entry Name:	ATG4D_HUMAN

Antibodies

Anti-ATG4D Antibody (CAB7842)

ATG4D Antibody (PACO05550)

ATG4D Antibody (PACO07905)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)