[KO Validated] Cytochrome C Monoclonal Antibody (CAB4912)
The [KO Validated] Cytochrome C Monoclonal Antibody (CAB4912) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, developed and validated specifically for use in Western blot applications, exhibits high reactivity with samples from various species, including humans.Cytochrome C is a key player in the intrinsic pathway of apoptosis, where it is released from the mitochondria in response to cellular stress, leading to programmed cell death.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] Cytochrome C Monoclonal Antibody
SKU:
CAB4912
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1153
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:5000 - 1:10000
IHC-P
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CYC, HCS, THC4, C
Positive Sample:
SH-SY5Y, A549, HuH-7, 4T1, NIH/3T3
Cellular Localization:
Mitochondrion Intermembrane Space.
Calculated MW:
12kDa
Observed MW:
14kDa
This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
Purification Method
Affinity purification
Gene ID
54205
RRID
AB_2863387
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cytochrome C Rabbit mAb (CAB4912) at 1:5000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Cytochrome C Rabbit mAb (CAB4912) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Cytochrome C Rabbit mAb (CAB4912) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Cytochrome C Rabbit mAb (CAB4912) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Cytochrome C Rabbit mAb (CAB4912) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Cytochrome C Rabbit mAb (CAB4912) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Cytochrome C Rabbit mAb (CAB4912) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Cytochrome C Rabbit mAb (CAB4912) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
