The [KO Validated] GSK3alpha Monoclonal Antibody (CAB19060) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to GSK3 alpha and has been rigorously validated for use in Western blot applications.GSK3 alpha is a serine/threonine kinase that is involved in multiple signaling pathways and has been implicated in various diseases including cancer, diabetes, and neurodegenerative disorders.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] GSK3alpha Monoclonal Antibody
SKU:
CAB19060
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0462
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.
Purification Method
Affinity purification
Gene ID
2931
RRID
AB_2862553
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and GSK3α knockout (KO) HeLa cells, using [KO Validated] GSK3α Rabbit mAb (CAB19060) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from Rat lung, using [KO Validated] GSK3α Rabbit mAb (CAB19060) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg GSK3α antibody (CAB19060). Western blot was performed from the immunoprecipitate using GSK3α antibody (CAB19060) at a dilution of 1:1000.
