The [KO Validated] HIF1AN/FIH1 Monoclonal Antibody (CAB3701) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, which is produced in rabbits, has been rigorously validated for knockout (KO) validation and is highly specific for detecting HIF1AN/FIH1 in various experimental settings.HIF1AN/FIH1 is a key regulator of the cellular response to hypoxia, playing a critical role in the stability and activity of HIF-1 alpha, a master transcription factor involved in the response to low oxygen levels.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KO Validated] HIF1AN/FIH1 Monoclonal Antibody
SKU:
CAB3701
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0826
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FIH1, H1
Positive Sample:
U-87MG, 293T
Cellular Localization:
Cytoplasm, Nucleus, Perinuclear Region.
Calculated MW:
41kDa
Observed MW:
40kDa
Enables several functions, including 2-oxoglutarate-dependent dioxygenase activity; NF-kappaB binding activity; and transition metal ion binding activity. Involved in several processes, including negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter in response to hypoxia; and protein hydroxylation. Located in cytosol; nucleoplasm; and perinuclear region of cytoplasm. Colocalizes with nucleus.
Purification Method
Affinity purification
Gene ID
55662
RRID
AB_2863123
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and HIF1AN/FIH1 knockout (KO) 293T cells, using [KO Validated] HIF1AN/FIH1 Rabbit mAb (CAB3701) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Western blot analysis of lysates from U-87MG cells, using HIF1AN/FIH1 Rabbit mAb (CAB3701) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
