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Anti-HIF1AN/FIH1 Antibody (CAB5466)[KO Validated]

SKU:
CAB5466
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • KO Validated Antibodies 1 Anti-HIF1AN/FIH1 Antibody CAB5466KO Validated
  • KO Validated Antibodies 1 Anti-HIF1AN/FIH1 Antibody CAB5466KO Validated
  • KO Validated Antibodies 1 Anti-HIF1AN/FIH1 Antibody CAB5466KO Validated
  • KO Validated Antibodies 1 Anti-HIF1AN/FIH1 Antibody CAB5466KO Validated
  • KO Validated Antibodies 1 Anti-HIF1AN/FIH1 Antibody CAB5466KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-HIF1AN/FIH1 Antibody [KO Validated]
Antibody SKU:CAB5466
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN/FIH1 (NP_060372.2).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, K-562, U-937, 293T, Mouse heart, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN/FIH1 (NP_060372.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAT AAEA VASG SGEP REEA GALG PAWD ESQL RSYS FPTR PIPR LSQS DPRA EELI ENEE PVVL TDTN LVYP ALKW DLEY LQEN IGNG DFSV YSAS THKF LYYD EKKM ANFQ NFKP RSNR EEMK FHEF VEKL QDIQ QRGG EERL YLQQ TLND TVGR KIVM DFLG FNWN WINK QQGK RGWG QLTS NLLL IGME GNVT PAHY DEQQ NFFA QIKG YKRC ILFP PDQF ECLY PYPV HHPC DRQS QVDF DNPD YERF PNFQ NVVG YETV VGPG DVLY IPMY WWHH IESL LNGG ITIT VNFW YKGA PTPK RIEY PLKA HQKV AIMR NIEK MLGE ALGN PQEV GPLL NTMI KGRY N
Gene ID:55662
Uniprot:Q9NWT6
Cellular Location:Cytoplasm, Nucleus, perinuclear region
Calculated MW:40kDa
Observed MW:40kDa
Synonyms:HIF1AN, FIH1
Background:
UniProt Protein Function:HIF1AN: Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. Homodimer; homodimerization is essential for catalytic activity. Interacts with VHL and HIF1A. Part of a complex with VHL, HIF1A and HDAC1 or HDAC2 or HDAC3. Interacts with NFKB1 and NFKBIA. Interacts with NOTCH1, NOTCH2 and NOTCH3 but not with NOTCH4. Interacts with APBA3; binding inhibits HIF1AN binding to HIF1A. Interacts with TNKS2. Interacts with PPP1R12A. Interacts with ASB4.
UniProt Protein Details:

Protein type:EC 1.14.11.30; EC 1.14.11.n4; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function:NF-kappaB binding; protein binding; protein homodimerization activity; oxygen sensor activity; zinc ion binding; carboxylic acid binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; Notch binding; cofactor binding

Biological Process: peptidyl-aspartic acid hydroxylation; transcription, DNA-dependent; peptidyl-asparagine hydroxylation; negative regulation of Notch signaling pathway; positive regulation of myoblast differentiation

UniProt Code:Q9NWT6
NCBI GenInfo Identifier:32129605
NCBI Gene ID:55662
NCBI Accession:Q9NWT6.2
UniProt Secondary Accession:Q9NWT6,Q5W147, Q969Q7, Q9NPV5, D3DR69,
UniProt Related Accession:Q9NWT6
Molecular Weight:40,285 Da
NCBI Full Name:Hypoxia-inducible factor 1-alpha inhibitor
NCBI Synonym Full Names:hypoxia inducible factor 1, alpha subunit inhibitor
NCBI Official Symbol:HIF1AN  
NCBI Official Synonym Symbols:FIH1  
NCBI Protein Information:hypoxia-inducible factor 1-alpha inhibitor; FIH-1; factor inhibiting HIF-1; factor inhibiting HIF1; hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase
UniProt Protein Name:Hypoxia-inducible factor 1-alpha inhibitor
UniProt Synonym Protein Names:Factor inhibiting HIF-1; FIH-1; Hypoxia-inducible factor asparagine hydroxylase
Protein Family:Hypoxia-inducible factor 1-alpha inhibitor
UniProt Gene Name:HIF1AN  
UniProt Entry Name:HIF1N_HUMAN
Anti-HIF1AN/FIH1 Antibody [KO Validated] (CAB5466)Western blot analysis of extracts of various cell lines, using HIF1AN/FIH1 antibody (CAB5466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-HIF1AN/FIH1 Antibody [KO Validated] (CAB5466)Western blot analysis of extracts from normal (control) and HIF1AN/FIH1 Rabbit pAb knockout (KO) 293T cells, using HIF1AN/FIH1 Rabbit pAb antibody (CAB5466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-HIF1AN/FIH1 Antibody [KO Validated] (CAB5466)Immunohistochemistry of paraffin-embedded rat brain using HIF1AN/FIH1 antibody (CAB5466) at dilution of 1:100 (40x lens).
Anti-HIF1AN/FIH1 Antibody [KO Validated] (CAB5466)Immunohistochemistry of paraffin-embedded human gastric cancer using HIF1AN/FIH1 antibody (CAB5466) at dilution of 1:100 (40x lens).
Anti-HIF1AN/FIH1 Antibody [KO Validated] (CAB5466)Immunohistochemistry of paraffin-embedded mouse heart using HIF1AN/FIH1 antibody (CAB5466) at dilution of 1:100 (40x lens).

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