The [KO Validated] MMP2 Monoclonal Antibody (CAB19080) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high specificity and sensitivity for MMP2 in human samples and has been rigorously validated for use in various applications such as Western blotting.MMP2 plays a crucial role in processes such as wound healing, angiogenesis, and tumor invasion, making it a key target for cancer research and therapeutic development. The ability of this antibody to specifically bind to MMP2 allows for accurate detection and analysis of its expression levels in different cell types and tissues, providing valuable insights into the role of MMP2 in various physiological and pathological conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] MMP2 Monoclonal Antibody
SKU:
CAB19080
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0432
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
4313
RRID
AB_2862572
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MMP2 Rabbit mAb (CAB19080) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
