Anti-NDUFB4 Antibody (CAB13820)

Antibody Name:	Anti-NDUFB4 Antibody [KO Validated]
Antibody SKU:	CAB13820
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HeLa, 293T, Mouse heart, Mouse kidney, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSFP KYKP SSLR TLPE TLDP AEYN ISPE TRRA QAER LAIR AQLK REYL LQYN DPNR RGLI ENPA LLRW AYAR TINV YPNF RPTP KNSL MG
Gene ID:	4710
Uniprot:	O95168
Cellular Location:	Matrix side, Mitochondrion inner membrane, Single-pass membrane protein
Calculated MW:	14kDa/15kDa
Observed MW:	15kDa

Synonyms:	NDUFB4, B15, CI-B15
Background:	This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone.

UniProt Protein Function:	NDUFB4: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB4 subunit family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; Energy Metabolism - oxidative phosphorylation; EC 1.6.5.3; EC 1.6.99.3; Membrane protein, integral; Oxidoreductase Chromosomal Location of Human Ortholog: 3q13.33 Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I; mitochondrion; nuclear membrane; nucleoplasm Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; response to oxidative stress
NCBI Summary:	This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone. [provided by RefSeq, Dec 2009]
UniProt Code:	O95168
NCBI GenInfo Identifier:	6093473
NCBI Gene ID:	4710
NCBI Accession:	O95168.3
UniProt Secondary Accession:	O95168,B2RUY3, B9EJC7,
UniProt Related Accession:	O95168
Molecular Weight:	14,026 Da
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH:ubiquinone oxidoreductase subunit B4
NCBI Official Symbol:	NDUFB4
NCBI Official Synonym Symbols:	B15; CI-B15
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
UniProt Synonym Protein Names:	Complex I-B15; CI-B15; NADH-ubiquinone oxidoreductase B15 subunit
Protein Family:	NADH dehydrogenase
UniProt Gene Name:	NDUFB4
UniProt Entry Name:	NDUB4_HUMAN

	Western blot analysis of extracts of various cell lines, using NDUFB4 antibody (CAB13820) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and NDUFB4 knockout (KO) HeLa cells, using NDUFB4 antibody (CAB13820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of L929 cells using NDUFB4 Polyclonal Antibody (CAB13820) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using NDUFB4 Polyclonal Antibody (CAB13820) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)