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[KO Validated] PHKG2 Rabbit Polyclonal Antibody (CAB19934)

SKU:
CAB19934
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] PHKG2 Rabbit Polyclonal Antibody
SKU:CAB19934
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 237-406 of human PHKG2 (NP_000285.1).
Sequence:RQIL MLRM IMEG QYQF SSPE WDDR SSTV KDLI SRLL QVDP EARL TAEQ ALQH PFFE RCEG SQPW NLTP RQRF RVAV WTVL AAGR VALS THRV RPLT KNAL LRDP YALR SVRH LIDN CAFR LYGH WVKK GEQQ NRAA LFQH RPPG PFPI MGPE EEGD SAAI TEDE AVLV LG
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:GSD9C; G2
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:cytosol, phosphorylase kinase complex
Calculated MW:46kDa
Observed MW:42kDa

Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Purification Method:Affinity purification
Gene ID:5261
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and PHKG2 knockout (KO) 293T cells, using [KO Validated] PHKG2 Rabbit pAb (CAB19934) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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