Anti-PHKG2 Antibody (CAB7337)

Antibody Name:	Anti-PHKG2 Antibody
Antibody SKU:	CAB7337
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 237-406 of human PHKG2 (NP_000285.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, 22RV1, HepG2, Jurkat, 293T, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 237-406 of human PHKG2 (NP_000285.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RQIL MLRM IMEG QYQF SSPE WDDR SSTV KDLI SRLL QVDP EARL TAEQ ALQH PFFE RCEG SQPW NLTP RQRF RVAV WTVL AAGR VALS THRV RPLT KNAL LRDP YALR SVRH LIDN CAFR LYGH WVKK GEQQ NRAA LFQH RPPG PFPI MGPE EEGD SAAI TEDE AVLV LG
Gene ID:	5261
Uniprot:	P15735
Cellular Location:
Calculated MW:	43kDa/46kDa
Observed MW:	46kDa

Synonyms:

PHKG2, GSD9C

Background:

Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

UniProt Protein Function:	PHKG2: a Ca(2 )/calmodulin-dependent protein kinase of the PHK family. Phosphorylase kinase is a polymer of 16 chains, four each of alpha, beta, gamma, and delta. Alpha and beta are regulatory chains, gamma is the catalytic chain, and delta is calmodulin. This isozyme may regulate glycogeneolysis in the testis.
UniProt Protein Details:	Protein type:EC 2.7.11.19; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; CAMK group; PHK family Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: phosphorylase kinase complex; cytosol Molecular Function:calmodulin binding; phosphorylase kinase activity; protein serine/threonine kinase activity; tau-protein kinase activity; ATP binding Biological Process: glycogen metabolic process; generation of precursor metabolites and energy; glycogen biosynthetic process; glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis; protein amino acid phosphorylation; positive regulation of glycogen catabolic process Disease: Glycogen Storage Disease Ixc
NCBI Summary:	Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]
UniProt Code:	P15735
NCBI GenInfo Identifier:	125536
NCBI Gene ID:	5261
NCBI Accession:	P15735.1
UniProt Secondary Accession:	P15735,P11800, A8K0C7, B4DEB7, E9PEU3,
UniProt Related Accession:	P15735
Molecular Weight:	406
NCBI Full Name:	Phosphorylase b kinase gamma catalytic chain, liver/testis isoform
NCBI Synonym Full Names:	phosphorylase kinase, gamma 2 (testis)
NCBI Official Symbol:	PHKG2
NCBI Official Synonym Symbols:	GSD9C
NCBI Protein Information:	phosphorylase b kinase gamma catalytic chain, liver/testis isoform; PSK-C3; PHK-gamma-T; PHK-gamma-LT; phosphorylase kinase subunit gamma-2; serine/threonine-protein kinase PHKG2; Phosphorylase kinase, gamma 2 (testis/liver); phosphorylase b kinase gamma catalytic chain, testis/liver isoform
UniProt Protein Name:	Phosphorylase b kinase gamma catalytic chain, liver/testis isoform
UniProt Synonym Protein Names:	PSK-C3; Phosphorylase kinase subunit gamma-2
Protein Family:	Phosphorylase b kinase
UniProt Gene Name:	PHKG2
UniProt Entry Name:	PHKG2_HUMAN

	Western blot analysis of extracts of various cell lines, using PHKG2 antibody (CAB7337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of C6 cells using PHKG2 Rabbit pAb (CAB7337) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using PHKG2 Rabbit pAb (CAB7337) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using PHKG2 Rabbit pAb (CAB7337) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using PHKG2 Rabbit pAb (CAB7337) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-PHKG2 Antibody (CAB14040)

Anti-PHKG2 Antibody (CAB19934)[KO Validated]

PHKG2 Antibody (PACO11218)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)