The [KO Validated] BAF57/SMARCE1 Antibody (CAB13353) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the SMARCE1 protein, this antibody allows for the detection and analysis of SMARCE1 in various cell types, making it ideal for studies in molecular biology and cancer research.SMARCE1, a member of the SWI/SNF chromatin remodeling complex, plays a critical role in regulating gene expression and cellular processes such as DNA repair and cell proliferation.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] BAF57/SMARCE1 Antibody
SKU:
CAB13353
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CSS5, BAF57, E1
Positive Sample:
293T
Cellular Localization:
Nucleus.
Calculated MW:
47kDa
Observed MW:
50kDa
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.
Purification Method
Affinity purification
Gene ID
6605
RRID
AB_2861685
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] BAF57/SMARCE1 Rabbit pAb (CAB13353) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and BAF57/SMARCE1 knockout (KO) 293T cells, using [KO Validated] BAF57/SMARCE1 Rabbit pAb (CAB13353) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunoprecipitation analysis of 150 μg extracts of Jurkat cells using 3 μg BAF57/BAF57/SMARCE1 antibody (CAB13353). Western blot was performed from the immunoprecipitate using BAF57/BAF57/SMARCE1 antibody (CAB13353) at a dilution of 1:1000.
