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[KO Validated] Vimentin Rabbit Monoclonal Antibody (CAB19607)

SKU:
CAB19607
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • KO Validated Antibodies 2 Anti-Vimentin Antibody KO Validated CAB19607
  • KO Validated Antibodies 2 Anti-Vimentin Antibody KO Validated CAB19607
  • KO Validated Antibodies 2 Anti-Vimentin Antibody KO Validated CAB19607
  • KO Validated Antibodies 2 Anti-Vimentin Antibody KO Validated CAB19607
  • KO Validated Antibodies 2 Anti-Vimentin Antibody KO Validated CAB19607
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] Vimentin Rabbit Monoclonal Antibody
SKU:CAB19607
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 367-466 of human Vimentin (P08670).
Sequence:DEIQ NMKE EMAR HLRE YQDL LNVK MALD IEIA TYRK LLEG EESR ISLP LPNF SSLN LRET NLDS LPLV DTHS KRTL LIKT VETR DGQV INET SQHH DDLE
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:WB,1:2000 - 1:20000 IHC-P,1:100 - 1:500 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:CTRCT30; HEL113; Vimentin; VIM; vimentin; in
Positive Sample:293T,Jurkat,HeLa,C6,Mouse testis,293T
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:54kDa
Observed MW:54kDa/

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Purification Method:Affinity purification
Gene ID:7431
Clone Number:ARC0086
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using [KD Validated] Vimentin Rabbit mAb (CAB19607) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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