The Kynurenine ELISA Kit from Assay Genie is specifically designed for the accurate measurement of kynurenine levels in various biological samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an invaluable tool for researchers in a wide range of applications. Kynurenine is a key metabolite in the tryptophan pathway and is implicated in a variety of physiological and pathological processes including inflammation, neurodegenerative diseases, and immune regulation.
By accurately quantifying kynurenine levels, researchers can gain valuable insights into disease mechanisms and identify potential therapeutic targets. Whether studying immune responses, neurological disorders, or inflammatory conditions, the Kynurenine ELISA Kit offers a reliable and efficient solution for measuring kynurenine levels with precision and accuracy. Stay ahead in your research with Assay Genie's Kynurenine ELISA Kit.
Product Name:
KYN (Kynurenine) ELISA Kit
SKU:
AEFI01031
Reactivity:
Universal
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
28.125 pmol/mL
Range:
46.875-3000 pmol/mL
Sample Type:
Cell Culture Supernatant, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
0
1
2
3
Cell Culture Supernatant (n = 5)
89-100%
87-100%
83-97%
Recovery:
0
1
2
Cell Culture Supernatant (n = 5)
95-103
100
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Lyophilized)
1 vial
1 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
