Anti-LRP5 Antibody (CAB0130)

Antibody Name:	Anti-LRP5 Antibody
Antibody SKU:	CAB0130
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1250 of human LRP5 (NP_002326.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1250 of human LRP5 (NP_002326.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IKRA KDDG TQPF VLTS LSQG QNPD RQPH DLSI DIYS RTLF WTCE ATNT INVH RLSG EAMG VVLR GDRD KPRA IVVN AERG YLYF TNMQ DRAA KIER AALD GTER EVLF TTGL IRPV ALVV DNTL GKLF WVDA DLKR IESC DLSG ANRL TLED ANIV QPLG LTIL GKHL YWID RQQQ MIER VEKT TGDK RTRI QGRV AHLT GIHA VEEV SLEE FSAH PCAR DNGG CSHI CIAK GDGT PRCS CPVH LVLL QNL
Gene ID:	4041
Uniprot:	O75197
Cellular Location:	Endoplasmic reticulum, Membrane, Single-pass type I membrane protein
Calculated MW:	179kDa
Observed MW:	150kDa

Synonyms:

LRP5, BMND1, EVR1, EVR4, HBM, LR3, LRP-5, LRP7, OPPG, OPS, OPTA1, VBCH2

Background:

This gene encodes a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with type 1 diabetes mellitus in humans. This protein plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in this gene. Mutations in this gene also cause familial exudative vitreoretinopathy. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	LRP5: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Appears be required for postnatal control of vascular regression in the eye. Required for posterior patterning of the epiblast during gastrulation. Homodimer; disulfide-linked. Forms phosphorylated oligomer aggregates on Wnt-signaling. Component of a Wnt-signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts with FZD8; the interaction is formed on WNT-binding and signaling. Interacts (via the phosphorylated PPPSP motif domains) with AXIN1; the interaction prevents inhibition of beta-catenin phosphorylation and signaling and is enhanced in the presence of GSK3B and WNT1 or WNT3A. Interacts (via beta-propeller regions 3 and 4) with DKK1; the interaction, enhanced by MESD and/or KREMEN, inhibits beta-catenin signaling by preventing GSK3-mediated phosphorylation of the PPPSP motifs and subsequent, AXIN1 binding. Interacts with MESD; the interaction prevents the formation of LRP5 aggregates, targets LRP5 to the plasma membrane and, when complexed with KREMEN2, increases DKK1 binding. Interacts with CSNK1E. Interacts with SOST; the interaction antagonizes canonical Wnt signaling. Interacts with APCDD1. Widely expressed, with the highest level of expression in the liver and in aorta. Belongs to the LDLR family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 11q13.4 Cellular Component: plasma membrane; receptor complex Molecular Function:protein binding; toxin transporter activity; Wnt receptor activity; Wnt-protein binding Biological Process: bone marrow development; cholesterol homeostasis; glucose catabolic process; negative regulation of osteoblast differentiation; osteoblast development; positive regulation of cell proliferation; positive regulation of fat cell differentiation; positive regulation of mesenchymal cell proliferation; positive regulation of mitosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of blood pressure; retina morphogenesis in camera-type eye; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin Disease: Bone Mineral Density Quantitative Trait Locus 1; Endosteal Hyperostosis, Autosomal Dominant; Exudative Vitreoretinopathy 1; Exudative Vitreoretinopathy 4; Osteopetrosis, Autosomal Dominant 1; Osteoporosis; Osteoporosis-pseudoglioma Syndrome; Van Buchem Disease, Type 2
NCBI Summary:	This gene encodes a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with type 1 diabetes mellitus in humans. This protein plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in this gene. Mutations in this gene also cause familial exudative vitreoretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:	O75197
NCBI GenInfo Identifier:	62512139
NCBI Gene ID:	4041
NCBI Accession:	O75197.2
UniProt Secondary Accession:	O75197,Q96TD6, Q9UES7, Q9UP66,
UniProt Related Accession:	O75197
Molecular Weight:	179,145 Da
NCBI Full Name:	Low-density lipoprotein receptor-related protein 5
NCBI Synonym Full Names:	LDL receptor related protein 5
NCBI Official Symbol:	LRP5
NCBI Official Synonym Symbols:	HBM; LR3; OPS; EVR1; EVR4; LRP7; OPPG; BMND1; LRP-5; OPTA1; VBCH2
NCBI Protein Information:	low-density lipoprotein receptor-related protein 5
UniProt Protein Name:	Low-density lipoprotein receptor-related protein 5
Protein Family:	Low-density lipoprotein receptor-related protein
UniProt Gene Name:	LRP5
UniProt Entry Name:	LRP5_HUMAN

	Western blot analysis of extracts of various cell lines, using LRP5 antibody (CAB0130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver using LRP5 antibody (CAB0130) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using LRP5 antibody (CAB0130) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using LRP5 antibody (CAB0130) at dilution of 1:200 (40x lens).

ELISA Kits

Human LRP5 ELISA Kit

Human Low-density lipoprotein receptor-related protein 5 (LRP5) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)