The Human LXA4 (Lipoxin A4) ELISA Kit is designed for the precise measurement of LXA4 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.LXA4 is a lipid mediator known for its anti-inflammatory properties and its role in regulating immune responses. It is involved in resolving inflammation and promoting tissue repair, making it a valuable biomarker for studying inflammatory diseases, autoimmune disorders, and other inflammatory conditions. By utilizing the Human LXA4 ELISA Kit, researchers can gain insights into the role of LXA4 in various physiological and pathological processes, ultimately advancing our understanding of inflammation and paving the way for potential therapeutic interventions.
Product Name:
LXA4 (Lipoxin A4) ELISA Kit
SKU:
UNES00034
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
