Anti-LY6E Antibody (CAB10225)
- SKU:
- CAB10225
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-LY6E Antibody |
| Antibody SKU: | CAB10225 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 21-101 of human LY6E (NP_002337.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | LO2, 293T, SKOV3, MCF7, U-87MG, Mouse brain, Mouse kidney, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 21-101 of human LY6E (NP_002337.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LMCF SCLN QKSN LYCL KPTI CSDQ DNYC VTVS ASAG IGNL VTFG HSLS KTCS PACP IPEG VNVG VASM GISC CQSF LCNF S |
| Gene ID: | 4061 |
| Uniprot: | Q16553 |
| Cellular Location: | Cell membrane, GPI-anchor, Lipid-anchor |
| Calculated MW: | 13kDa |
| Observed MW: | 14kDa |
| Synonyms: | LY6E, RIG-E, RIGE, SCA-2, SCA2, TSA-1 |
| Background: |
| UniProt Protein Function: | LY6E: Induced by retinoic acid; in promyelocytic leukemia NB4 and in myeloblast HL-60 cell lines. Activated by IFN-alpha in monocytic cell line U-937 and in peripheral blood monocyte cells. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: integral to plasma membrane Biological Process: epinephrine secretion; organ growth; cell surface receptor linked signal transduction; in utero embryonic development; adrenal gland development; ventricular cardiac muscle morphogenesis; norepinephrine metabolic process |
| UniProt Code: | Q16553 |
| NCBI GenInfo Identifier: | 4505049 |
| NCBI Gene ID: | 4061 |
| NCBI Accession: | NP_002337.1 |
| UniProt Secondary Accession: | Q16553,Q0VDE5, B2R4X5, D3DWJ2, |
| UniProt Related Accession: | Q16553 |
| Molecular Weight: | 13,507 Da |
| NCBI Full Name: | lymphocyte antigen 6E |
| NCBI Synonym Full Names: | lymphocyte antigen 6 complex, locus E |
| NCBI Official Symbol: | LY6EÂ Â |
| NCBI Official Synonym Symbols: | RIGE; SCA2; RIG-E; SCA-2; TSA-1Â Â |
| NCBI Protein Information: | lymphocyte antigen 6E |
| UniProt Protein Name: | Lymphocyte antigen 6E |
| UniProt Synonym Protein Names: | Retinoic acid-induced gene E protein; RIG-E; Stem cell antigen 2; SCA-2; Thymic shared antigen 1 |
| Protein Family: | Lymphocyte antigen |
| UniProt Gene Name: | LY6EÂ Â |
| UniProt Entry Name: | LY6E_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LY6E antibody (CAB10225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of L929 cells using LY6E Rabbit pAb (CAB10225) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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