Description
Lysozyme for bacterial lysis
Lysozyme is a high-purity enzyme for lysing bacterial cell walls by hydrolysing peptidoglycan, used to prepare bacterial lysates for nucleic acid and protein extraction. It is supplied as a dry powder with its own Lysozyme Buffer for easy preparation of a 20 mg/ml working solution.
Lysozyme is an alkaline enzyme sourced from egg white. It cleaves the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the bacterial cell wall, breaking insoluble mucopolysaccharides into soluble glycopeptides. This ruptures the cell wall and lyses the bacteria. It is mainly used to digest Gram-positive bacterial cell walls during DNA and RNA preparation. For research use only.
Key Features
| Efficient cell wall lysis | Cleaves β-1,4 glycosidic bonds in peptidoglycan to rupture bacterial cell walls. | |
| Ideal for Gram-positive bacteria | Mainly used to digest Gram-positive cell walls before DNA and RNA preparation. | |
| Buffer included | Dissolve the powder in the supplied Lysozyme Buffer to make a 20 mg/ml working solution. |
Specifications
| Source | Egg white |
| Format | Dry powder with Lysozyme Buffer |
| Mechanism | Hydrolyses β-1,4 glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine |
| Application | Digestion of Gram-positive bacterial cell walls for DNA and RNA preparation |
| Working concentration | 20 mg/ml (20 mg in 1 ml Lysozyme Buffer) |
| Shelf life | 2 years |
Components
| Components | Amount |
| Lysozyme | 200 mg |
| Lysozyme Buffer | 10 ml |
Storage
Store at 2 ~ 8°C.
- Prepare the working solution
- Dissolve 20 mg of lysozyme powder in 1 ml of Lysozyme Buffer to make a 20 mg/ml working solution.
- Store any unused solution
- Aliquot the working solution and store at -20°C.
Q1: How do I prepare the lysozyme working solution?
- Dissolve 20 mg of lysozyme powder in 1 ml of the supplied Lysozyme Buffer to obtain a 20 mg/ml working solution.
Q2: Can I store the prepared lysozyme solution?
- Fresh preparation is recommended. If not used immediately, aliquot the solution and store at -20°C, avoiding repeated freeze-thaw cycles so that activity is not affected.
Q3: Which bacteria is lysozyme used for?
- It is mainly used to digest the cell walls of Gram-positive bacteria during DNA and RNA preparation.
