Anti-MAP1LC3A Antibody (CAB11438)

Antibody Name:	Anti-MAP1LC3A Antibody
Antibody SKU:	CAB11438
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP1LC3A (NP_115903.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Rat heart, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP1LC3A (NP_115903.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPSD RPFK QRRS FADR CKEV QQIR DQHP SKIP VIIE RYKG EKQL PVLD KTKF LVPD HVNM SELV KIIR RRLQ LNPT QAFF LLVN QHSM VSVS TPIA DIYE
Gene ID:	84557
Uniprot:	Q9H492
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Calculated MW:	14kDa
Observed MW:	18kDa

Synonyms:

MAP1LC3A, ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3

Background:

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

UniProt Protein Function:	LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Microtubule-binding; Ubiquitin-like modifier; Autophagy; Vesicle Chromosomal Location of Human Ortholog: 20q11.22 Cellular Component: microtubule; extrinsic to membrane; late endosome; autophagic vacuole; cytoplasmic vesicle; cytosol Molecular Function:protein binding; phosphatidylethanolamine binding; microtubule binding; GABA receptor binding; phospholipid binding Biological Process: mitochondrion degradation; cellular response to nitrogen starvation; autophagic vacuole formation
NCBI Summary:	MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]
UniProt Code:	Q9H492
NCBI GenInfo Identifier:	85701362
NCBI Gene ID:	84557
NCBI Accession:	Q9H492.2
UniProt Secondary Accession:	Q9H492,Q9GZQ8,
UniProt Related Accession:	Q9H492
Molecular Weight:
NCBI Full Name:	Microtubule-associated proteins 1A/1B light chain 3A
NCBI Synonym Full Names:	microtubule associated protein 1 light chain 3 alpha
NCBI Official Symbol:	MAP1LC3A
NCBI Official Synonym Symbols:	LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
NCBI Protein Information:	microtubule-associated proteins 1A/1B light chain 3A
UniProt Protein Name:	Microtubule-associated proteins 1A/1B light chain 3A
UniProt Synonym Protein Names:	Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha
UniProt Gene Name:	MAP1LC3A
UniProt Entry Name:	MLP3A_HUMAN

	Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody (CAB11438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using MAP1LC3A antibody (CAB11438) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using MAP1LC3A antibody (CAB11438) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-MAP1LC3A Antibody (CAB12319)	Human MAP1LC3A ELISA Kit
	Human Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)