Anti-MAP2K3 Antibody (CAB7718)

Antibody Name:	Anti-MAP2K3 Antibody
Antibody SKU:	CAB7718
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human MAP2K3 (NP_659731.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human MAP2K3 (NP_659731.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MESP ASSQ PASM PQSK GKSK RKKD LRIS CMSK PPAP NPTP PRNL DSRT FITI GDRN FEVE ADDL VTIS ELGR GAYG VVEK VRHA QSGT IMAV KRIR ATVN SQEQ KRLL MDLD INMR TVDC FYTV TFYG ALFR EGDV WICM ELMD TSLD KFYR KVLD KNMT IPED ILGE IA
Gene ID:	5606
Uniprot:	P46734
Cellular Location:
Calculated MW:	36kDa/39kDa
Observed MW:	39kDa

Synonyms:

MAP2K3, MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2

Background:

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.

UniProt Protein Function:	Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
NCBI Summary:	The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P46734
NCBI GenInfo Identifier:	24638466
NCBI Gene ID:	5606
NCBI Accession:	P46734.2
UniProt Secondary Accession:	P46734,Q99441, Q9UE71, Q9UE72, B3KSK7,
UniProt Related Accession:	P46734
Molecular Weight:	39,940 Da
NCBI Full Name:	Dual specificity mitogen-activated protein kinase kinase 3
NCBI Synonym Full Names:	mitogen-activated protein kinase kinase 3
NCBI Official Symbol:	MAP2K3
NCBI Official Synonym Symbols:	MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
NCBI Protein Information:	dual specificity mitogen-activated protein kinase kinase 3
UniProt Protein Name:	Dual specificity mitogen-activated protein kinase kinase 3
UniProt Synonym Protein Names:	MAPK/ERK kinase 3; MEK 3; Stress-activated protein kinase kinase 2; SAPK kinase 2; SAPKK-2; SAPKK2
Protein Family:	Dual specificity mitogen-activated protein kinase kinase
UniProt Gene Name:	MAP2K3

	Western blot analysis of extracts of various cell lines, using MAP2K3 antibody (CAB7718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat ovary using MAP2K3 antibody (CAB7718) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using MAP2K3 antibody (CAB7718) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using MAP2K3 antibody (CAB7718) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using MAP2K3 antibody (CAB7718) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Phospho-MAP2K3 (S218) Antibody (PACO00230)	MKK3 (Phospho-Ser189) Colorimetric Cell-Based ELISA Kit
Phospho-MAP2K3 (T222) Antibody (PACO04763)	MKK3 Colorimetric Cell-Based ELISA Kit
MAP2K3 Antibody (PACO04764)	MKK3 (Phospho-Thr222) Colorimetric Cell-Based ELISA Kit
MAP2K3 Antibody (PACO06632)
MAP2K3 Antibody (PACO07467)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)