Anti-MDC1 Antibody (CAB8358)

Antibody Name:	Anti-MDC1 Antibody
Antibody SKU:	CAB8358
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1 (NP_055456.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	Jurkat, LO2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1 (NP_055456.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEDT QAID WDVE EEEE TEQS SESL RCNV EPVG RLHI FSGA HGPE KDFP LHLG KNVV GRMP DCSV ALPF PSIS KQHA EIEI LAWD KAPI LRDC GSLN GTQI LRPP KVLS PGVS HRLR DQEL ILFA DLLC QYHR LDVS LPFV SRGP LTVE ETPR VQGE TQPQ RLLL AEDS EEEV DFLS ERRM VKKS RTTS SSVI VPES DEEG HSPV LGGL GPPF AFNL NSDT DVEE GQQP ATEE ASSA ARRG ATVE AKQS EAEV VTEI QLEK DQPL VKER DNDT KVKR GAGN GVVP AGVI LERS QPPG EDSD TDVD DDSR PPGR PAEV HLER AQPF GFID SDTD AEEE RIPA TPVV IPMK KR
Gene ID:	9656
Uniprot:	Q14676
Cellular Location:	Chromosome, Nucleus
Calculated MW:	116kDa/195kDa/197kDa/226kDa
Observed MW:	270kDa

Synonyms:

MDC1, NFBD1

Background:

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

UniProt Protein Function:	MDC1: Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 6p21.33 Cellular Component: chromosome; focal adhesion; nuclear body; nucleoplasm; nucleus Molecular Function:protein binding; protein C-terminus binding Biological Process: double-strand break repair via nonhomologous end joining; intra-S DNA damage checkpoint
NCBI Summary:	The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008]
UniProt Code:	Q14676
NCBI GenInfo Identifier:	68565390
NCBI Gene ID:	9656
NCBI Accession:	Q14676.3
UniProt Secondary Accession:	Q14676,Q0EFC2, Q2L6H7, Q2TAZ4, Q5JP55, Q5JP56, Q5ST83 A2AB04, A2BF04, A2RRA8, A7YY86, B0S8A2,
UniProt Related Accession:	Q14676
Molecular Weight:	227kDa
NCBI Full Name:	Mediator of DNA damage checkpoint protein 1
NCBI Synonym Full Names:	mediator of DNA damage checkpoint 1
NCBI Official Symbol:	MDC1
NCBI Official Synonym Symbols:	NFBD1
NCBI Protein Information:	mediator of DNA damage checkpoint protein 1
UniProt Protein Name:	Mediator of DNA damage checkpoint protein 1
UniProt Synonym Protein Names:	Nuclear factor with BRCT domains 1
Protein Family:	Mediator of DNA damage checkpoint protein
UniProt Gene Name:	MDC1

	Western blot analysis of extracts of various cell lines, using MDC1 antibody (CAB8358) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human lung cancer using MDC1 antibody (CAB8358) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using MDC1 antibody (CAB8358) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-MDC1 Antibody (CAB12714)

Phospho-MDC1 (S513) Antibody (PACO05798)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)