Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (STMA00055). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (STMA00055) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT.
MDC1 Antibody [P2B11] is a mouse monoclonal antibody directed against MDC1, offered by Assay Genie for research applications. Reported applications include WB and ICC/IF. Reported reactivity: Human, Mouse, Bovine and Chimpanzee. Supplied as IgG1, purified by Protein G.
Mediator of DNA Damage Checkpoint 1 (MDC1) is a key regulator of the DNA damage response (DDR), orchestrating the recruitment of repair proteins to sites of double-strand breaks. In post-mitotic neurons, where genomic integrity is critical for long-term function, MDC1 plays a vital role in maintaining neuronal viability. Dysregulation of MDC1 impairs DNA repair efficiency, contributing to the accumulation of DNA lesions observed in neurodegenerative diseases such as Alzheimer’s and Huntington’s disease. MDC1 also interacts with chromatin remodeling complexes and checkpoint kinases, linking it to broader epigenetic and cell cycle control mechanisms. As DNA damage and genomic instability are emerging hallmarks of neurodegeneration, MDC1 represents a promising target for therapeutic strategies aimed at enhancing neuronal resilience and genomic maintenance. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Product Name:
MDC1 Antibody [P2B11]
Product SKU:
STMA00055
Size:
100 µg
Target:
MDC1
Host Species:
Mouse
Antibody Type:
Monoclonal
Clone ID:
P2B11
Research Areas:
Cancer, Oxidative Stress, Cell Signaling, Epigenetics and Nuclear Signaling
Buffer:
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage & Shipping:
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, ICC/IF
Antibody Isotype:
IgG1
Purification:
Protein G Purified
Concentration:
1 mg/ml
Reactivity:
Human, Mouse, Bovine, Chimpanzee
Recommended Dilution:
WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Specificity:
Detects ~184kDa. This antibody recognizes MDC1 at and around the N-terminus.
Guarantee:
12 months from date of dispatch
Immunogen:
GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
Immunogen Species:
Mouse
Accession Number:
NP_001010833.2
Gene ID:
240087
Swiss-Prot:
Q5PSV9
Cellular Localization:
Nucleus
Alternative Names:
MDC1, Nuclear factor with BRCT domains1, Mediator of DNA damage checkpoint 1
References:
1. Lou Z., et al. (2004) J Biol Chem. 279(45): 46359-46362. 2. Luo K., Yuan J, Lou Z. (2011) J Biol Chem. 286(32): 28192-28199. 3. Strauss C., Halevy T., Macarov M., Argaman L., and Goldbery M. (2011) DNA Repair (Amst.). 10(8): 806-814. 4. Wilson K.A., et al. (2011) Mol Cancer Res. 9(6): 766-781.
