The MEK1/MEK2 Monoclonal Antibody (CAB4868) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] RRID AB_2863368 Gene ID 5604 5605 Swiss Prot Synonym CFC3; MAPKK1; MEK1; MKK1; PRKMK1; MEK1/MEK2
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MEK1/MEK2 Monoclonal Antibody
SKU:
CAB4868
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0292
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:5000 - 1:30000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MEK1/MEK2
Positive Sample:
HeLa, Hep G2, Jurkat, Mouse brain
Cellular Localization:
Cytosol, Early Endosome, Endoplasmic Reticulum, Focal Adhesion, Golgi Apparatus, Late Endosome, Microtubule Organizing Center, Mitochondrion, Nucleus, Plasma Membrane.
Calculated MW:
44kDa
Observed MW:
44kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] RRID AB_2863368 Gene ID 5604 5605 Swiss Prot Synonym CFC3; MAPKK1; MEK1; MKK1; PRKMK1; MEK1/MEK2
Purification Method:
Affinity purification
Gene ID:
5604 5605
RRID:
AB_2863368
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MEK1/MEK2 Rabbit mAb (CAB4868) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
