Anti-MGLL Antibody (CAB6654)

Antibody Name:	Anti-MGLL Antibody
Antibody SKU:	CAB6654
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human MGLL (NP_009214.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, A-549, SH-SY5Y, Mouse heart, Rat brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human MGLL (NP_009214.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	METG PEDP SSMP EESS PRRT PQSI PYQD LPHL VNAD GQYL FCRY WKPT GTPK ALIF VSHG AGEH SGRY EELA RMLM GLDL LVFA HDHV GHGQ SEGE RMVV SDFH VFVR DVLQ HVDS MQKD YPGL PVFL LGHS MGGA IAIL TAAE RPGH FAGM VLIS PLVL ANPE SATT FKVL AAKV LNLV LPNL SLGP IDSS VLSR NKTE VDIY NSDP LICR AGLK VCFG IQLL NAVS RVER ALPK LTVP FLLL QGSA DRLC DSKG AYLL MELA KSQD KTLK IYEG AYHV LHKE LPEV TNSV FHEI NMWV SQR
Gene ID:	11343
Uniprot:	Q99685
Cellular Location:	Cytoplasm, Membrane, Peripheral membrane protein, cytosol
Calculated MW:	31kDa/33kDa
Observed MW:	33KDa

Synonyms:

MGLL, HU-K5, HUK5, MAGL, MGL

Background:

This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	MGLL: Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.
UniProt Protein Details:	Protein type:Phospholipase; EC 3.1.1.23; Lipid Metabolism - glycerolipid Chromosomal Location of Human Ortholog: 3q21.3 Cellular Component: nucleoplasm; endoplasmic reticulum membrane; extrinsic to membrane; plasma membrane; synapse; cytosol Molecular Function:protein homodimerization activity; acylglycerol lipase activity; lipid binding; lysophospholipase activity Biological Process: platelet activation; glycerophospholipid biosynthetic process; regulation of signal transduction; regulation of axon extension; phospholipid metabolic process; triacylglycerol catabolic process; regulation of inflammatory response; arachidonic acid metabolic process; lipid metabolic process; regulation of sensory perception of pain; inflammatory response; blood coagulation; fatty acid biosynthetic process; acylglycerol catabolic process
NCBI Summary:	This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
UniProt Code:	Q99685
NCBI GenInfo Identifier:	47117287
NCBI Gene ID:	11343
NCBI Accession:	Q99685.2
UniProt Secondary Accession:	Q99685,Q6IBG9, Q96AA5, B3KRC2, B7Z9D1,
UniProt Related Accession:	Q99685
Molecular Weight:	303
NCBI Full Name:	Monoglyceride lipase
NCBI Synonym Full Names:	monoglyceride lipase
NCBI Official Symbol:	MGLL
NCBI Official Synonym Symbols:	MGL; HUK5; MAGL; HU-K5
NCBI Protein Information:	monoglyceride lipase; monoacylglycerol lipase; lysophospholipase homolog
UniProt Protein Name:	Monoglyceride lipase
UniProt Synonym Protein Names:	HU-K5; Lysophospholipase homolog; Lysophospholipase-like; Monoacylglycerol lipase; MAGL
Protein Family:	Monoglyceride lipase
UniProt Gene Name:	MGLL
UniProt Entry Name:	MGLL_HUMAN

	Western blot analysis of extracts of various cell lines, using MGLL antibody (CAB6654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Western blot analysis of extracts of Mouse heart, using MGLL antibody (CAB6654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.

Antibodies	ELISA Kits
MGLL Antibody (PACO10482)	Human Monoacylglycerol Lipase / MAG lipase ELISA Kit
	Human MGL (Monoacylglycerol Lipase) CLIA Kit (HUES00373)
	Human MGL (Monoacylglycerol Lipase) ELISA Kit (HUES01728)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)