The Mouse VCAM-1/CD106 (Vascular cell adhesion molecule 1) ELISA (Small Sample Volume) is designed for the quantitative detection of Mouse VCAM-1/CD106 (Vascular cell adhesion molecule 1) levels in various biological samples. Adenosine Monophosphate (AMP) and related adenosine species are critical purine nucleotides that function as cellular energy indicators and signaling molecules. AMP activates AMP-activated protein kinase (AMPK), the master regulator of cellular energy homeostasis, when ATP levels fall and AMP:ATP ratios increase. Measurement of adenosine-related metabolites and their interconversion enzymes is fundamental to understanding energy metabolism, purinergic signaling, immunomodulation, and disease states including ischemia, diabetes, and cancer. Assay Genie's Mouse VCAM-1/CD106 (Vascular cell adhesion molecule 1) ELISA (Small Sample Volume) offers exceptional sensitivity and specificity with a sensitivity of 46.88 pg/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Mouse VCAM-1/CD106 (Vascular cell adhesion molecule 1) ELISA (Small Sample Volume) for accurate and dependable quantification of this crucial biomarker in your studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 2 vials, 20 mL | 96T*5: 10 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
90-104
89-104
91-102
Average (%)
95
95
96
1:4
Range (%)
84-100
89-104
86-98
Average (%)
91
96
92
1:8
Range (%)
91-104
86-100
87-102
Average (%)
96
93
94
1:16
Range (%)
89-100
89-101
92-102
Average (%)
95
95
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
86-99
92
Cell Culture Media (n=8)
85-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
262.86
566.26
2221.13
255.82
616.54
2117.35
Standard deviation
13.46
38.0
91.29
20.24
46.8
189.29
C V (%)
5.12
6.71
4.11
7.91
7.59
8.94
Uniport ID:
P29533
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 25 μL
Specificity:
This kit recognizes Mouse VCAM-1/CD106 in samples.No significant cross-reactivity or interference between Mouse VCAM-1/CD106 and analogues was observed
