Human VCAM-1/CD106 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit
The Human VCAM-1 (CD106) ELISA Kit is specially designed for the precise measurement of Vascular Cell Adhesion Molecule-1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research purposes.Vascular Cell Adhesion Molecule-1 (VCAM-1) is a key molecule involved in cellular adhesion and inflammation, playing a crucial role in various diseases such as atherosclerosis, inflammation, and autoimmune disorders. By detecting VCAM-1 levels, researchers can gain valuable insights into the pathogenesis of these conditions and potentially identify new therapeutic targets.Overall, the Human VCAM-1 (CD106) ELISA Kit is a valuable tool for studying the role of VCAM-1 in disease mechanisms and developing novel treatment strategies.
Product Name:
Human VCAM-1/CD106 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit
SKU:
HUES03559
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 2 vials, 20 mL | 96T*5: 10 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
94-106
98-109
90-107
Average (%)
100
103
98
1:4
Range (%)
91-102
85-99
82-97
Average (%)
97
91
89
1:8
Range (%)
90-105
82-93
88-99
Average (%)
96
87
93
1:16
Range (%)
92-105
82-95
81-94
Average (%)
99
88
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
94-105
100
Cell Culture Media (n=8)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.88
11.94
36.46
5.21
11.28
34.49
Standard deviation
0.25
0.67
2.35
0.38
0.96
2.26
C V (%)
5.18
5.64
6.45
7.27
8.53
6.55
Uniport ID:
P19320
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Human VCAM-1/CD106 and analogues was observed
