The Mouse VCAM-1 (Vascular Cell Adhesion Molecule-1) ELISA Kit is specially designed for the precise measurement of VCAM-1 levels in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent tool for a variety of research applications.VCAM-1 is a key protein involved in mediating cell adhesion and inflammation, playing a vital role in various physiological and pathological processes. Its expression is upregulated in conditions such as atherosclerosis, inflammatory diseases, and cancer, highlighting its importance as a biomarker for studying these diseases and potentially developing targeted therapies.Overall, the Mouse VCAM-1 ELISA Kit offers researchers a reliable and effective method for quantifying VCAM-1 levels in mouse samples, providing valuable insights into the role of this protein in health and disease.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 2 vials, 20 mL | 96T*5: 10 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
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Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
92-105
100-115
93-104
Average (%)
100
106
98
1:4
Range (%)
98-110
86-100
94-109
Average (%)
104
92
102
1:8
Range (%)
94-105
84-97
94-111
Average (%)
100
90
101
1:16
Range (%)
97-115
85-96
95-112
Average (%)
105
91
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
85-95
90
Cell Culture Media (n=8)
88-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
0.87
3.59
0.55
0.87
3.41
Standard deviation
0.03
0.04
0.24
0.04
0.06
0.23
C V (%)
5.47
4.93
6.56
8.01
7.4
6.89
Uniport ID:
P29533
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Mouse VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Mouse VCAM-1/CD106 and analogues was observed
