Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit
The Rat VCAM-1 (Vascular Cell Adhesion Molecule-1) ELISA Kit is specifically designed for the accurate measurement of VCAM-1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and dependable results for a variety of research applications.VCAM-1 is a key protein involved in cell adhesion and plays a critical role in inflammatory responses and immune cell recruitment. Elevated levels of VCAM-1 have been linked to various inflammatory diseases, making it a valuable biomarker for studying these conditions and potential therapeutic targets. With its reliable performance and ease of use, the Rat VCAM-1 ELISA Kit is essential for researchers striving to understand the role of VCAM-1 in disease pathogenesis and developing novel interventions. Purchase this kit today to advance your research in the field of inflammation and vascular biology.
Product Name:
Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit
SKU:
RTES00881
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
7.5 pg/mL
Detection range:
12.5-800 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
