Anti-MMP17 Antibody (CAB3030)

Antibody Name:	Anti-MMP17 Antibody
Antibody SKU:	CAB3030
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human MMP17

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, RAW264.7, Mouse testis, Rat testis

Immunogen:	A synthetic peptide of human MMP17
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4326
Uniprot:	Q9ULZ9
Cellular Location:	Cell membrane, Extracellular side, GPI-anchor, Lipid-anchor, Secreted, extracellular matrix, extracellular space
Calculated MW:	57kDa/66kDa
Observed MW:	75kDa

Synonyms:

MMP17, MMP-17, MT4-MMP, MT4MMP, MTMMP4

Background:

This gene encodes a member of the peptidase M10 family and membrane-type subfamily of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Members of this subfamily contain a transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. The encoded preproprotein is proteolytically processed to generate the mature protease. This protein is unique among the membrane-type matrix metalloproteinases in that it is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. Elevated expression of the encoded protein has been observed in osteoarthritis and multiple human cancers.

UniProt Protein Function:	MMP17: Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin. Belongs to the peptidase M10A family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Activator; EC 3.4.24.-; Membrane protein, integral; Membrane protein, GPI anchor; Protease Chromosomal Location of Human Ortholog: 12q24.3 Cellular Component: integral to plasma membrane Molecular Function:enzyme activator activity
NCBI Summary:	This gene encodes a member of the peptidase M10 family and membrane-type subfamily of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Members of this subfamily contain a transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. The encoded preproprotein is proteolytically processed to generate the mature protease. This protein is unique among the membrane-type matrix metalloproteinases in that it is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. Elevated expression of the encoded protein has been observed in osteoarthritis and multiple human cancers. [provided by RefSeq, Jan 2016]
UniProt Code:	Q9ULZ9
NCBI GenInfo Identifier:	296439485
NCBI Gene ID:	4326
NCBI Accession:	Q9ULZ9.4
UniProt Secondary Accession:	Q9ULZ9,Q14850,
UniProt Related Accession:	Q9ULZ9
Molecular Weight:	57,938 Da
NCBI Full Name:	Matrix metalloproteinase-17
NCBI Synonym Full Names:	matrix metallopeptidase 17
NCBI Official Symbol:	MMP17
NCBI Official Synonym Symbols:	MMP-17; MT4MMP; MTMMP4; MT4-MMP
NCBI Protein Information:	matrix metalloproteinase-17
UniProt Protein Name:	Matrix metalloproteinase-17
UniProt Synonym Protein Names:	Membrane-type matrix metalloproteinase 4; MT-MMP 4; MTMMP4; Membrane-type-4 matrix metalloproteinase; MT4-MMP; MT4MMP
Protein Family:	Matrix metalloproteinase
UniProt Gene Name:	MMP17
UniProt Entry Name:	MMP17_HUMAN

	Western blot analysis of extracts of various cell lines, using MMP17 Rabbit pAb (CAB3030) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded Human colon using MMP17 Rabbit pAb (CAB3030) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse brain using MMP17 Rabbit pAb (CAB3030) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Cleaved-MMP17 (Q129) Antibody (PACO00035)	Human MMP17 ELISA Kit
	Human MMP-17 (Matrix Metalloproteinase 17) CLIA Kit (HUES00824)
	Human Matrix metalloproteinase-17 (MMP17) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)