Description
Mouse Atherosclerosis Array (22 targets) (SARB0075)
The Mouse Atherosclerosis Array (SARB0075) is a comprehensive research tool designed for the study of atherosclerosis, a common cardiovascular disease characterized by the buildup of plaque in the arteries. This array includes 22 targets related to atherosclerosis, allowing researchers to analyze various aspects of the disease process.Designed for use with mouse samples, this array enables the simultaneous detection of multiple biomarkers involved in atherosclerosis, providing a comprehensive overview of the molecular pathways and mechanisms underlying the disease. By studying the expression levels of these targets, researchers can gain valuable insights into the pathogenesis of atherosclerosis and identify potential therapeutic targets for intervention.
The Mouse Atherosclerosis Array is a valuable tool for researchers working in the fields of cardiovascular biology, vascular disease, and lipid metabolism. With its high sensitivity and specificity, this array offers a reliable and efficient platform for studying the complex molecular processes involved in atherosclerosis development and progression. Whether investigating disease mechanisms, biomarker discovery, or drug development, this array provides a versatile and powerful resource for advancing research in the field of cardiovascular disease.
| Product Sku: | SARB0075 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 22 |
| Gene Symbols: | CCL11, CCL2, CCL20, CCL5, CD40, CSF1, CSF2, CSF3, FGF2, IFNG|IL13, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, SELL, SELP, TNF, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
bFGF
| CD40 (TNFRSF5)
| Eotaxin-1 (CCL11)
| GCSF
| GM-CSF
|
IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-3
|
IL-4
| IL-5
| IL-6
| IL-13
| L-Selectin (CD62L)
|
MCP-1 (CCL2)
| M-CSF
| MIP-3 alpha (CCL20)
| P-Selectin
| RANTES (CCL5)
|
TNF alpha
| VEGF-A
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Atherosclerosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
