The Mouse Col1 (Collagen Type I) CLIA Kit is a highly accurate and reliable assay designed for the detection of collagen type I levels in mouse samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Collagen type I is a key component of the extracellular matrix and plays a vital role in tissue structure, function, and repair. Abnormal collagen type I levels have been associated with various diseases, including fibrosis, arthritis, and other connective tissue disorders. Therefore, this kit serves as a valuable tool for investigating collagen type I dynamics in disease progression and potential therapeutic interventions.With its user-friendly protocol and high-performance characteristics, the Mouse Col1 (Collagen Type I) CLIA Kit is an essential resource for researchers studying the complex role of collagen type I in health and disease. Visit the website for more information on this innovative assay kit.
Product Name:
Mouse COL1 (Collagen Type I) CLIA Kit
SKU:
MOES00193
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
