The Mouse Col1 (Collagen Type I) ELISA Kit is specifically designed for the precise measurement of Collagen Type I levels in mouse serum, plasma, and tissue culture samples. With its high sensitivity and specificity, this kit delivers accurate and reliable results, making it a valuable tool for researchers in various fields.Collagen Type I is a fundamental component of the extracellular matrix, playing a crucial role in tissue structure, strength, and integrity. Its dysregulation is associated with various diseases, including fibrosis, arthritis, and skin disorders. Therefore, this ELISA kit provides researchers with a valuable tool to study Collagen Type I levels and its implications in disease development and progression.Overall, the Mouse Col1 (Collagen Type I) ELISA Kit offers researchers a reliable and efficient method to quantitatively measure Collagen Type I levels in mouse samples, leading to a better understanding of its role in health and disease.
Product Name:
Mouse COL1 (Collagen Type I) ELISA Kit
SKU:
MOES00888
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
