The Rat COL1 (Collagen Type 1) CLIA Kit is specifically designed for the quantitative measurement of collagen type 1 levels in rat samples such as serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Collagen type 1 is a key component of the extracellular matrix and plays a crucial role in maintaining tissue structure and integrity. Abnormal collagen type 1 levels have been associated with various diseases and conditions, including fibrosis, arthritis, and osteoporosis.
Therefore, this kit serves as a valuable tool for studying collagen metabolism and potential therapeutic interventions in these disorders.Overall, the Rat COL1 (Collagen Type 1) CLIA Kit provides researchers with a reliable and efficient solution for investigating collagen type 1 levels in rat samples, ultimately advancing our understanding of tissue remodeling and disease pathogenesis.
Product Name:
Rat COL1 (Collagen Type 1) CLIA Kit
SKU:
RTES00136
Target:
Rat COL1 (Collagen Type 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat COL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat COL1. You can calculate the concentration of Rat COL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
88-103
86-100
Average (%)
92
95
93
1:4
Range (%)
92-108
101-117
84-96
Average (%)
100
108
91
1:8
Range (%)
95-108
89-99
101-119
Average (%)
101
94
109
1:16
Range (%)
93-105
94-106
85-98
Average (%)
100
100
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.0
2.42
8.25
1.05
2.61
7.78
Standard deviation
0.1
0.25
0.7
0.11
0.28
0.84
C V (%)
10.0
10.33
8.48
10.48
10.73
10.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat COL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COL1 in samples. No significant cross-reactivity or interference between Rat COL1 and analogues was observed.
