Human COL1 alpha1 (Collagen Type I Alpha 1) CLIA Kit
The Human COL1A1 (Collagen Type I Alpha 1) CLIA Kit is specially designed for the precise measurement of collagen type I alpha 1 levels in human samples like serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Collagen type I alpha 1 is a key component of the extracellular matrix and is essential for maintaining the structural integrity and strength of tissues such as skin, bones, and tendons. Dysregulation of collagen type I alpha 1 is associated with various diseases, including fibrosis, osteoporosis, and connective tissue disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. Whether you are investigating tissue repair mechanisms, studying fibrotic diseases, or exploring potential treatments for bone disorders, the Human COL1A1 CLIA Kit provides a reliable tool to accurately quantify collagen type I alpha 1 levels and advance your research efforts.
Product Name:
Human COL1 alpha1 (Collagen Type I Alpha 1) CLIA Kit
SKU:
HUES00524
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
