Human COL1 alpha1 (Collagen Type I Alpha 1) ELISA Kit
The Human Collagen Type I Alpha 1 ELISA Kit is specially designed for the precise measurement of collagen type I alpha 1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, guaranteeing accurate and consistent results, which makes it perfect for a variety of research purposes.Collagen type I alpha 1 is a key component of collagen, a structural protein that plays a crucial role in maintaining the integrity and strength of tissues such as skin, bone, and tendons. Changes in collagen type I alpha 1 levels have been linked to various health conditions, including skin disorders, osteoporosis, and connective tissue diseases, making it a valuable biomarker for studying these diseases and developing potential treatments. For researchers looking to explore the role of collagen type I alpha 1 in health and disease, the Human Collagen Type I Alpha 1 ELISA Kit provides a reliable and effective tool for accurate quantification and analysis.
Product Name:
Human COL1 alpha1 (Collagen Type I Alpha 1) ELISA Kit
SKU:
HUES01997
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
