Human COL3 alpha1 (Collagen Type III Alpha 1) ELISA Kit
The Human Col3A1 (Collagen Type III Alpha 1) ELISA Kit is specifically designed for the precise measurement of collagen type III alpha 1 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research purposes.Collagen type III alpha 1 is a key component of the extracellular matrix, playing a vital role in tissue structure, repair, and remodeling. Dysregulation of collagen type III alpha 1 has been implicated in various diseases such as fibrosis, connective tissue disorders, and cardiovascular conditions, highlighting its importance as a biomarker for disease progression and therapeutic development.By utilizing the Human Col3A1 ELISA Kit, researchers can gain valuable insights into the regulation of collagen type III alpha 1 and its implications in disease pathology, paving the way for innovative diagnostic and treatment strategies.
Product Name:
Human COL3 alpha1 (Collagen Type III Alpha 1) ELISA Kit
SKU:
HUES01916
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
