Human COL2 alpha1 (Collagen Type II Alpha 1) ELISA Kit
The Human Collagen Type II Alpha 1 (COL2A1) ELISA Kit is specifically designed for the accurate detection of COL2A1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.COL2A1 is a key structural protein found in cartilage and plays a critical role in maintaining the integrity and function of connective tissues. Dysregulation of COL2A1 expression is associated with various musculoskeletal disorders, such as osteoarthritis and rheumatoid arthritis. Therefore, this ELISA kit serves as a valuable tool for studying these conditions and potentially developing therapeutic interventions.Overall, the Human Collagen Type II Alpha 1 (COL2A1) ELISA Kit provides researchers with a reliable and effective method for quantifying COL2A1 levels, facilitating advancements in the understanding and treatment of connective tissue-related diseases.
Product Name:
Human COL2 alpha1 (Collagen Type II Alpha 1) ELISA Kit
SKU:
HUES01915
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
