Rat COL1 alpha2 (Collagen Type I Alpha 2) CLIA Kit
The Rat COL1A2 (Collagen Type I Alpha 2) CLIA Kit is specifically designed for the quantitative measurement of rat COL1A2 levels in cell lysates and tissue homogenates. This kit offers high sensitivity and specificity, providing accurate and reproducible results for various research applications.COL1A2 is a key component of collagen, the most abundant protein in the extracellular matrix, essential for the structural integrity of tissues and organs. Abnormal levels of COL1A2 have been linked to various pathological conditions, including fibrosis, arthritis, and osteoporosis, making it a crucial biomarker for studying these diseases and potential therapeutic interventions.
Overall, the Rat COL1A2 CLIA Kit offers researchers a reliable tool for investigating the role of collagen type I alpha 2 in health and disease, paving the way for advancements in the understanding and treatment of collagen-related disorders.
Product Name:
Rat COL1 alpha2 (Collagen Type I Alpha 2) CLIA Kit
SKU:
RTES00140
Target:
Rat COL1 alpha2 (Collagen Type I Alpha 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat COL1α2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL1α2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL1α2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat COL1α2. You can calculate the concentration of Rat COL1α2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
86-100
92-104
Average (%)
91
92
99
1:4
Range (%)
99-114
100-118
93-108
Average (%)
105
107
99
1:8
Range (%)
101-119
100-117
91-108
Average (%)
109
107
99
1:16
Range (%)
85-99
90-101
98-114
Average (%)
92
96
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
104
EDTA plasma (n=5)
93-109
100
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.07
306.22
822.44
102.11
285.27
858.33
Standard deviation
9.14
22.45
63.82
10.12
31.29
86.35
C V (%)
8.87
7.33
7.76
9.91
10.97
10.06
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat COL1α2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COL1α2 in samples. No significant cross-reactivity or interference between Rat COL1α2 and analogues was observed.
