The Mouse GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit is a reliable and accurate tool for the detection of GM-CSF levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides consistent and reproducible results, making it suitable for a variety of research applications.GM-CSF is a key cytokine involved in the regulation of immune cell development and function, playing a role in inflammatory responses, immune system function, and hematopoiesis. Its dysregulation has been linked to various diseases, including autoimmune disorders, inflammatory conditions, and cancer. Therefore, the Mouse GM-CSF ELISA kit is an essential tool for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse GM-CSF ELISA Kit from Assay Genie is a valuable resource for researchers seeking to investigate the role of GM-CSF in health and disease, providing accurate and reliable data for further exploration and understanding in the field of immunology and beyond.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
