The Porcine GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit is designed for the quantitative detection of Porcine GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) levels in various biological samples. Osteocalcin (OC/Bone Gla Protein/BGP) is a small, vitamin K-dependent protein synthesized exclusively by osteoblasts and is the most abundant non-collagenous protein in bone matrix. Following synthesis, osteocalcin undergoes gamma-carboxylation by vitamin K-dependent enzymes, enabling its binding to hydroxyapatite in bone mineral. Serum osteocalcin concentrations reflect the rate of bone formation and osteoblast activity, making it a specific biochemical marker of bone turnover widely used in metabolic bone disease research, osteoporosis monitoring, and assessment of anabolic therapy responses. Assay Genie's Porcine GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit offers exceptional sensitivity and specificity with a sensitivity of 0.91 pg/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Porcine GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
