Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit
The Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit is a specialized assay designed for the quantitative detection of GM-CSF levels in rat biological samples. GM-CSF is a crucial cytokine that plays a pivotal role in the regulation of hematopoiesis and immune responses, particularly in stimulating the growth and differentiation of granulocytes and macrophages. This ELISA kit offers exceptional sensitivity and specificity, ensuring the accurate measurement of GM-CSF concentrations in rat samples. GM-CSF is an essential factor in leukocyte production and immune function, making it a key target for research in immunology, hematopoiesis, and inflammatory conditions. Manufactured under rigorous quality control standards, this ELISA kit provides robust performance, reliable results, and ease of use, making it an excellent choice for researchers studying GM-CSF and its impact on immune regulation and hematopoiesis in rats.
Product Name:
Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit
SKU:
AEES00291
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
