The Mouse GPX4 (Phospholipid Hydroperoxide Glutathione Peroxidase) ELISA Kit is specifically designed for the quantitative measurement of GPX4 levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications. GPX4 is a key enzyme that plays a critical role in protecting cells from oxidative damage and lipid peroxidation. It is essential for maintaining cellular homeostasis and preventing cell death. Dysregulation of GPX4 has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, highlighting its significance as a potential biomarker for disease progression and therapeutic intervention.
By utilizing the Mouse GPX4 ELISA Kit, researchers can gain valuable insights into the role of GPX4 in disease pathogenesis and explore its potential as a therapeutic target. This kit provides a convenient and accurate tool for studying GPX4 biology and its implications in various pathological conditions.
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-102%
95-98%
87-101%
EDTA Plasma (n = 5)
85-100%
82-99%
84-101%
Heparin Plasma (n = 5)
85-98%
80-94%
80-94%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-103
95
EDTA Plasma (n = 5)
90-100
99
Heparin Plasma (n = 5)
91-95
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
